Characterisation of pathogen-specific biomarkers in bacterial pneumonia using high-capacity sorptive extraction (HiSorb™) and automated chemometrics

Species-specific volatile organic compounds (VOCs) secreted by bacteria hold promise for use as biomarkers, enabling rapid, non-invasive determination of bacterial disease by gas chromatography–mass spectrometry (GC–MS).

Headspace HiSorb high-capacity sorptive extraction was used to extract analytes from four different bacterial cultures, followed by thermal desorption (TD)–GC–MS analysis. Subsequent chemometric analysis of the data using ChromCompare+ enabled quick identification of unique VOCs in each species, enabling the four bacterial species to be easily distinguished computationally based on their VOC profiles. Three characteristic VOCs per species were confirmed as having potential for use as biomarkers.

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