Simple, non-invasive sampling of short-chain fatty acids in saliva as potential indicators of disease using HiSorb sorptive extraction
The Chemistry Lab for Analytical Technologies and Sensors, Dept. of Chemistry, University of Pisa, Italy
A team from the Department of Chemistry and Industrial Chemistry of the University of Pisa, Italy, undertook a study working with saliva samples collected from four patient groups (acute HF, chronic HF, obese, and hypertensive) using HiSorb™ high-capacity sorptive extraction. Markes International supplied the HiSorb probes for the study.
The determination of short-chain fatty acids and hydroxy acids in saliva is challenging due to their relatively low concentrations as well as their hydrophilic nature and limited volatility. Most LC- and GC-based protocols require time-consuming and labour-intensive analytical workflows.
In this case study, we talk to Dr Tommaso Lomonaco about how the team developed a reliable, simple, and organic solvent-free protocol to address and provide solutions to these challenges.
- Customer: University of Pisa, Italy
- Application: Analysis of saliva samples
- Challenge: To develop and validate an analytical workflow for the simultaneous determination of short-chain fatty acids, branched-chain fatty acids and lactic acid in human saliva and evaluate their suitability as non-invasive indicators of gut and oral dysbiosis
- Solution: HiSorb™ high-capacity sorptive extraction
- Results: Acid variations related to HF rather than comorbidity